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Nakamura et al., 2019)

There are three primary mechanisms by which CLAs are thought to reduce inflammation and oxidative stress: (1) CLA competes with linoleic acid in production of arachidonic acid, which itself is a precursor to prostaglandin E2, and thus CLA reduces the production of prostaglandins, by affecting the expression of cyclooxygenase 2 enzyme [62]

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For examination of cytokines and cellular proliferation, conjugated antibodies against any combination of the following intracellular antigens were used: TNF (MP6-X122, 1:200), IFN (XMG1.2, 1:200) and Ki-67(16A8, 1:1,000)